Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - bone
Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - lung
Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - liver
Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - Human Kidney
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Retina
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Retina
Get tips on using VWR Life Science RiboZol™ RNA Extraction Reagent to perform RNA isolation / purification Cells - primary rat brain microvascular endothelial cells
Get tips on using EZ1 DNA Tissue Kit (48) to perform DNA isolation / purification Tissue - FFPE samples
Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
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