siRNA / RNAi /miRNA transfection Rat A-10

Get tips on using PACE4 siRNA (h) to perform siRNA / miRNA gene silencing Human - DU145 PACE4

Get tips on using Hs_GLI1_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - DAOY GLI1

Get tips on using BECN1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - BV2 BECN1

Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Colon26 CD98

Get tips on using Hs_HDAC5_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - U937 HDAC5

Get tips on using CD151 siRNA (h) to perform siRNA / miRNA gene silencing Human - U251 CD151

Get tips on using FAK siRNA (h) to perform siRNA / miRNA gene silencing Human - U251 FAK

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - Rat Brain

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using ATG5 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - NIH-3T3 Atg5