DNA methylation profiling Gene specific profiling Human ovarian tissue

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

Get tips on using Neuron specific enolase monoclonal antibody (47) to perform Immunohistochemistry Mouse - NSE

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Ovarian Stem Cells

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Ovarian CSC

Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - mouse kidney tissue