DNA transfection Mammalian cells Primary cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human mononuclear cells

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary hematopoietic stem cells

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary hematopoietic stem cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human endothelial cells

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary porcine primary chondrocytes

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary canine peripheral blood mononuclear cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood mononuclear cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat brain microvascular endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit aortic smooth muscle cells