rna-isolation-purification-cells-primary-human-carotid-artery-endothelial-cells

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized Caov-3

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized MDAH 2774

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized OVCAR-3

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized SKOV-3

Get tips on using MagExtractor -RNA to perform RNA isolation / purification Cells - immortalized COV-434

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780-CIS

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Stem cell culture media Mice BMSC

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Mammalian cell culture media CADMEC/HMVEC

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to be considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.