siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2)

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Notch 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - A2780 Notch 1

Get tips on using YAP siRNA (h) to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene Lipofectamine

Get tips on using AllStars Hs Cell Death siRNA to perform siRNA / miRNA gene silencing Human - U2OS KRAS

Get tips on using SMARTpool: ON-TARGETplus TP63 siRNA to perform siRNA / miRNA gene silencing Human - A253 P36

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - HT-1376 GLUT1

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - HT-1376 CD74

Get tips on using Gβ 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D GNB1

Get tips on using Stealth siRNA™ NOTCH2 to perform siRNA / miRNA gene silencing Human - THP-1 NOTCH2

Get tips on using Stealth siRNA™ NOTCH1 to perform siRNA / miRNA gene silencing Human - THP-1 NOTCH1