Cell Culture Contamination Detection Kit

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity

Get tips on using CHO Media to perform Mammalian cell culture media CHO

Get tips on using HEK GM to perform Mammalian cell culture media HEK

Get tips on using Medium 199 to perform Mammalian cell culture media PAOSMC

Get tips on using Advanced DMEM to perform Mammalian cell culture media HC

Get tips on using Medium 231 to perform Mammalian cell culture media HAOSMC

Get tips on using Medium 200 to perform Mammalian cell culture media HAOECv

Get tips on using Medium 231 to perform Mammalian cell culture media BPAEC