Cell line Authentication kit

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - mouse splenocytes

Get tips on using ApopTag Fluorescein in Situ Apoptosis Detection Kit to perform TUNEL assay cell type - HEK293 human embryonic kidney cells

Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - PA-1

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - MG-63

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - OECM-1

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - THP-1

Get tips on using Annexin V-FITC Early Apoptosis Detection Kit to perform Apoptosis assay cell type - HT-29

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - HT-29

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - CaOV-3