Flowcytometry CD14 Mouse / IgG2a

Get tips on using CD115 (c-fms) Monoclonal Antibody (AFS98), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD115

Get tips on using CD11b Monoclonal Antibody (M1/70), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD11b

Get tips on using CD137 (4-1BB) Monoclonal Antibody (17B5), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD137

Get tips on using ALCAM Antibody (B-6): sc-74558 to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using PE anti-human CD114 (G-CSFR) Antibody to perform Flow cytometry Anti-bodies Human - CD114

Get tips on using PE/Dazzle™ 594 anti-human CD184 (CXCR4) Antibody to perform Flow cytometry Anti-bodies Human - CD184/CXCR4

Get tips on using PDGFR-α Antibody (C-9): sc-398206 to perform Flow cytometry Anti-bodies Human - CD140/PDFGR2

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using CD24 Antibody (M1/69): sc-19651 to perform Immunohistochemistry Mouse - CD24

Get tips on using Recombinant Anti-CD34 antibody [EP373Y] (ab81289) to perform Immunohistochemistry Mouse - CD34