Immunohistochemistry ATM phospho Rabbit IgG

Get tips on using Attractene Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines OVCAR-3

Get tips on using ATG5 Antibody (N-term) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Attractene Transfection Reagent to perform shRNA gene silencing Human - HEK 293T CAPN5- (Calpains) cationic lipid based

Get tips on using Atg12 Antibody (Mouse Specific) #2011 to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Monoclonal Anti-ATG5 antibody produced in mouse to perform Autophagy assay cell type - CaCo-2

Get tips on using Monoclonal Anti-ATG12 antibody produced in mouse to perform Autophagy assay cell type - CaCo-2

Get tips on using McCoy's 5A Medium, ATCC® 30-2007™ to perform Mammalian cell culture media A253

Get tips on using Iscove's Modified Dulbecco's Medium (IMDM) (ATCC® 30-2005™) to perform Mammalian cell culture media KG1

Get tips on using MTT Cell Proliferation Assay (ATCC® 30-1010K™) to perform Cell cytotoxicity / Proliferation assay cell type - MCF-7

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.