Western blot 1,4 β-N-acetyl-D-glucosamine Triticum vulgaris

Get tips on using RPMI 1640 to perform Mammalian cell culture media HepG2

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Get tips on using RPMI 1640 to perform Mammalian cell culture media CHO

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

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TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media HUVEC

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media BEnEpC