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shRNA gene silencing

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Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates CHO

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates H9C2

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / miRNA gene silencing Human - siRNA negative control Lipid

Products Thermo Fisher Scientific Lipofectamine® RNAiMAX Transfection Reagent

Get tips on using STEAP2 metalloreductase to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) STEAP2

Products Thermo Fisher Scientific STEAP2 metalloreductase

Get tips on using Hs_TET3_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 TET3(TET methylcytosine dioxygenase 3)

Products Qiagen Hs_TET3_2 FlexiTube siRNA

Get tips on using OCT4-PG1 siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) OCT4-PG1

Products Thermo Fisher Scientific OCT4-PG1 siRNA

Get tips on using siRNA MAPKAPK-2 to perform siRNA / miRNA gene silencing Human - U937 MK2 (MAPK Kinase 2) Viral vectors

Products Santa Cruz Biotechnology siRNA MAPKAPK-2

Get tips on using siRNA MAPKAPK-2 to perform siRNA / miRNA gene silencing Human - Jurkat MK2 (MAPK Kinase 2) Viral vectors

Products Santa Cruz Biotechnology siRNA MAPKAPK-2

Get tips on using HIF-1α siRNA (r) to perform siRNA / miRNA gene silencing Rat - Brain endothelial cells HIF-1α Lipid

Products Santa Cruz Biotechnology HIF-1α siRNA (r)

Get tips on using Silencer® Select- Gdf10 siRNA to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 BMP-3b/GDF10

Products Thermo Fisher Scientific Silencer® Select- Gdf10 siRNA

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