3D Cell Culture Media Human breast cancer MCF-7 cells-Mammospheres

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Found 3 matching solutions for this experiment

DMEM/F-12, no phenol red

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	Seed 3000 cells/well (MCF7) Use of pHEMA (Sigma) -coated 6-well plates Score of mammospheres > 50μm

Protocol tips
Seed 3000 cells/well (MCF7) Use of pHEMA (Sigma) -coated 6-well plates Score of mammospheres > 50μm

Publication protocol 1 Relevant paper

MammoCult™ Human Medium Kit

STEMCELL technologies

Upstream tips	Protocol tips	Downstream tips
	Seed at a density of 5,000 cells/mL Use 6-well ultra-low-attachment plates Count mammospheres ≥50 μm in diameter

Protocol tips
Seed at a density of 5,000 cells/mL Use 6-well ultra-low-attachment plates Count mammospheres ≥50 μm in diameter

Manufacturer protocol Publication protocol 1 Relevant paper

MEBMTM Mammary Epithelial Cell Growth Basal Medium

Lonza

Upstream tips	Protocol tips	Downstream tips
	Seed of cells at several densities (10, 50, 100, 1000 cells per well) Use of 96-well ultra-low attachment plates Count of >50μm mammospheres

Protocol tips
Seed of cells at several densities (10, 50, 100, 1000 cells per well) Use of 96-well ultra-low attachment plates Count of >50μm mammospheres

Manufacturer protocol Publication protocol 1 Relevant paper

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