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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| You can develop chemoresistant cell lines |
Seed 1.0 ×10 4 cells/well
Use of 24-ultralow attachment plates
Analysis of spheres with a diameter >40 μm |
Collect spheres for other procedures, such as RNA isolation |
| Upstream tips |
| You can develop chemoresistant cell lines |
| Protocol tips |
Seed 1.0 ×10 4 cells/well
Use of 24-ultralow attachment plates
Analysis of spheres with a diameter >40 μm |
| Downstream tips |
| Collect spheres for other procedures, such as RNA isolation |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Seed 1x10 3 cells/well |
|
| Protocol tips |
| Seed 1x10 3 cells/well |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Seed at a density of 5,000 cells/mL
Use 6-well ultra-low-attachment plates
Count mammospheres ≥50 μm in diameter |
|
| Protocol tips |
Seed at a density of 5,000 cells/mL
Use 6-well ultra-low-attachment plates
Count mammospheres ≥50 μm in diameter |
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