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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Use early passages (1–5) of primary tumor tissues from PDX models |
Seed PDX cells at a density of 500 cells/well
Use of 96-well ultralow attachment plates
Count spheres 5-7 days later |
Collect spheres for other procedures, such as RNA isolation |
| Upstream tips |
| Use early passages (1–5) of primary tumor tissues from PDX models |
| Protocol tips |
Seed PDX cells at a density of 500 cells/well
Use of 96-well ultralow attachment plates
Count spheres 5-7 days later |
| Downstream tips |
| Collect spheres for other procedures, such as RNA isolation |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Use of 6-well plate with an ultra-low attachment surface
Seeding of 10x103 sorted cells
When seeding of sorted cells, the plate should be left undisturbed for 5 days at 37°C
|
|
| Protocol tips |
Use of 6-well plate with an ultra-low attachment surface
Seeding of 10x103 sorted cells
When seeding of sorted cells, the plate should be left undisturbed for 5 days at 37°C
|
| Upstream tips |
Protocol tips |
Downstream tips |
| Use of cells isolated from a cryopreserved PDX tumor pieces |
Seed 5 × 103 cells derived from PDX
Use 24-well ultra-low-adherence plates
|
|
| Upstream tips |
| Use of cells isolated from a cryopreserved PDX tumor pieces |
| Protocol tips |
Seed 5 × 103 cells derived from PDX
Use 24-well ultra-low-adherence plates
|
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