Apoptosis assay cell type - L02

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 2 matching solutions for this experiment

Annexin V-FITC Apoptosis Detection Kit

Vazyme

Upstream tips	Protocol tips	Downstream tips
- Store all components at 4-8℃ and protect from light. - The Annexin V-FITC can be stored at -20℃ for longer use.	- After adding Annexin V-FITC and PI Staining Solution cells were Incubated at room temperature for 10 min and protect from light.

Upstream tips
- Store all components at 4-8℃ and protect from light. - The Annexin V-FITC can be stored at -20℃ for longer use.
Protocol tips
- After adding Annexin V-FITC and PI Staining Solution cells were Incubated at room temperature for 10 min and protect from light.

Manufacturer protocol Publication protocol 1 Relevant paper

FITC Annexin V Apoptosis Detection Kit I (RUO)

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Manufacturer protocol Publication protocol 1 Relevant paper

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