Autophagy assay cell type - KG1 cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

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Found 1 matching solution for this experiment

Atg3 Antibody

Cell Signaling Technology

Upstream tips	Protocol tips	Downstream tips
	Dilute Ab at 1:100 and incubate overnight at 4 °C

Protocol tips
Dilute Ab at 1:100 and incubate overnight at 4 °C

Manufacturer protocol Publication protocol 1 Relevant paper

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