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Found 6 matching solutions for this experiment
|Produces high-quality cDNA from nanograms of total or poly A+ RNA.
Store RNA and SMART II A Oligo at –70°C.
Store all other reagents at –20°C
|For ds cDNA Polishing Proteinase K treatment is necessary to inactivate the DNA polymerase activity before proceeding with the ligation steps.
Do not chill the tube at –20°C or on ice before centrifuging. Chilling the sample will result in coprecipitation of impurities
|Heat Oligo mixture to 65°C for 5 min and quick chill on ice and later add reaction components.
Mix and incubate at 42°C for 2 min.
Add 1 μL (200 units) of SuperScript™ II RT and mix by pipetting gently up and down.
Incubate at 42°C for 50 min.
Inactivate the reaction by heating at 70°C for 15 min.