Cell cycle assay human - AGS cell line

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 2 matching solutions for this experiment

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- After seeding,cells are treated without or with FCP at concentrations of 75 and 150 µg/ml for 24 h at 37°C. - For or 3 h at −20°C, cells are fixed in cold 70% ethanol (v/v)

Protocol tips
- After seeding,cells are treated without or with FCP at concentrations of 75 and 150 µg/ml for 24 h at 37°C. - For or 3 h at −20°C, cells are fixed in cold 70% ethanol (v/v)

Manufacturer protocol Publication protocol 1 Relevant paper

Cell Cycle Phase Determination Kit

Cayman Chemicals

Upstream tips	Protocol tips	Downstream tips
- Cells were seeded and treated with concentrations of the EtOAc fraction for 12 h.	- Cells were fixed and incubated for more than 2h.

Upstream tips
- Cells were seeded and treated with concentrations of the EtOAc fraction for 12 h.
Protocol tips
- Cells were fixed and incubated for more than 2h.

Manufacturer protocol Publication protocol 1 Relevant paper

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