Cell cycle assay human - HeLa

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

DNA-Prep Reagents Kit, RUO 1

Beckman Coulter

Upstream tips	Protocol tips	Downstream tips
- cells were seeded at 5×104 cells/ml and treated with IC50 values of AFha, AFe and Cha and incubated for 16 and 32 h	- 100 µl lysis reagent were added to each tube and incubated for 5 min at room temperature.

Upstream tips
- cells were seeded at 5×104 cells/ml and treated with IC50 values of AFha, AFe and Cha and incubated for 16 and 32 h
Protocol tips
- 100 µl lysis reagent were added to each tube and incubated for 5 min at room temperature.

Manufacturer protocol Publication protocol 1 Relevant paper

Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells are labelled with EdU for 20min	- For the detection of EdU with Alexa Fluor® 647 azide use 633/635 nm excitation with a red emission filter (660/20 nm or similar).

Protocol tips
- Cells are labelled with EdU for 20min
Downstream tips
- For the detection of EdU with Alexa Fluor® 647 azide use 633/635 nm excitation with a red emission filter (660/20 nm or similar).

Manufacturer protocol Publication protocol 1 Relevant paper

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Cells are incubated with securinine at concentrations of 10.0 and 20.0 μg/ml for 24 h and 48 h. DMSO concentration added to the cells was 0.2% (v/v)

Protocol tips
- Cells are incubated with securinine at concentrations of 10.0 and 20.0 μg/ml for 24 h and 48 h. DMSO concentration added to the cells was 0.2% (v/v)

Manufacturer protocol Publication protocol 1 Relevant paper

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