Cell cycle assay human - HT-29

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

BD Cycletest™ Plus DNA Kit

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
- Cells were seeded and treated with 2,4′,6-trihydroxy-4-methoxybenzophenone (at IC50) at 37°C for 24 h, 48 h, and 72 h.	- Cells were fixed with ethanol, washed in warm PBS, and incubated at −20°C, overnight

Upstream tips
- Cells were seeded and treated with 2,4′,6-trihydroxy-4-methoxybenzophenone (at IC50) at 37°C for 24 h, 48 h, and 72 h.
Protocol tips
- Cells were fixed with ethanol, washed in warm PBS, and incubated at −20°C, overnight

Manufacturer protocol Publication protocol 1 Relevant paper

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- After seeding cells are treated with 0.1% DMSO as a vehicle control or with various concentrations of EESR for 48 hours. - Cells are fixed with cold 70% ethanol for 3 hours at −20°C

Protocol tips
- After seeding cells are treated with 0.1% DMSO as a vehicle control or with various concentrations of EESR for 48 hours. - Cells are fixed with cold 70% ethanol for 3 hours at −20°C

Manufacturer protocol Publication protocol 1 Relevant paper

Cell Cycle and Apoptosis Analysis Kit

Beyotime

Upstream tips	Protocol tips	Downstream tips
	- Cells were treated with 20 µg/mL RNase, stained with 20 µg/mL propidium iodide

Protocol tips
- Cells were treated with 20 µg/mL RNase, stained with 20 µg/mL propidium iodide

Manufacturer protocol Publication protocol 1 Relevant paper

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