Cell cycle assay human - Jurkat

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

PI/RNase Staining Buffer

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
- Cells were were incubated with various concentrations of 1 (0, 25, 50, 75, 100, 150 and 200 µM); 2 (200 µM); 3 (200 µM); 4 (200 µM); 5 (500 µM); a mixture of ManNAc (125 µM) and 5 (500 µM); and ManNAc (10 mM) for five days.	- Fixed with 78% aqueous ethanol and incubated for 24h

Upstream tips
- Cells were were incubated with various concentrations of 1 (0, 25, 50, 75, 100, 150 and 200 µM); 2 (200 µM); 3 (200 µM); 4 (200 µM); 5 (500 µM); a mixture of ManNAc (125 µM) and 5 (500 µM); and ManNAc (10 mM) for five days.
Protocol tips
- Fixed with 78% aqueous ethanol and incubated for 24h

Manufacturer protocol Publication protocol 1 Relevant paper

PI/RNASE Solution

Immunostep

Upstream tips	Protocol tips	Downstream tips
- Cells (1×106 cells/well) were incubated with vehicle or 1-(3,4,5-trihydroxyphenyl)-dodecylbenzoate at their respectively IC50.	- After 24 h of incubation cells were treated with the protocol

Upstream tips
- Cells (1×106 cells/well) were incubated with vehicle or 1-(3,4,5-trihydroxyphenyl)-dodecylbenzoate at their respectively IC50.
Protocol tips
- After 24 h of incubation cells were treated with the protocol

Manufacturer protocol Publication protocol 1 Relevant paper

Guava Cell Cycle Reagent for Flow Cytometry

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	-Cells were stained with Guava Cell-Cycle Reagent that contains the dye PI	- Cells were analysed for cell cycle after every 24, 48 and 72 h treatment with reagent

Protocol tips
-Cells were stained with Guava Cell-Cycle Reagent that contains the dye PI
Downstream tips
- Cells were analysed for cell cycle after every 24, 48 and 72 h treatment with reagent

Manufacturer protocol Publication protocol 1 Relevant paper

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