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Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
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- Cells were labelled with 10 μmol/L of 5-ethynyl-2′-deoxyuridine and incubated for 60 minutes at 37°C.
- Cells were fixed with 4% paraformaldehyde for 60 minutes instead of 15min.
- 7-AAD was added for measuring DNA content and cell cycle distribution. |
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Protocol tips |
- Cells were labelled with 10 μmol/L of 5-ethynyl-2′-deoxyuridine and incubated for 60 minutes at 37°C.
- Cells were fixed with 4% paraformaldehyde for 60 minutes instead of 15min.
- 7-AAD was added for measuring DNA content and cell cycle distribution. |
Upstream tips |
Protocol tips |
Downstream tips |
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- Cells are fixed with 70% ethanol at 4°C for 24 h.
- Cells are stained with 50 μg/mL of PI and 100 μg/mL of RNase A at 37°C for 30 min in the dark.
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Protocol tips |
- Cells are fixed with 70% ethanol at 4°C for 24 h.
- Cells are stained with 50 μg/mL of PI and 100 μg/mL of RNase A at 37°C for 30 min in the dark.
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Upstream tips |
Protocol tips |
Downstream tips |
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- If low level of staining is noticed, increase volume of stain used. |
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Protocol tips |
- If low level of staining is noticed, increase volume of stain used. |
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