Cell cycle assay human - SKOV3

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Guava Cell Cycle Reagent for Flow Cytometry

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
- Cells were cultured, seeded and later centrifuged at 450 × g for 5 minutes, and fixed with ethanol.

Upstream tips
- Cells were cultured, seeded and later centrifuged at 450 × g for 5 minutes, and fixed with ethanol.

Manufacturer protocol Publication protocol 1 Relevant paper

Cell Cycle and Apoptosis Analysis Kit

Beyotime

Upstream tips	Protocol tips	Downstream tips
		- The red fluorescence was detected by a flow cytometer at an excitation wavelength of 488 nm.

Downstream tips
- The red fluorescence was detected by a flow cytometer at an excitation wavelength of 488 nm.

Manufacturer protocol Publication protocol 1 Relevant paper

Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
- This assay was used for determining the percentage of S-phase cells

Upstream tips
- This assay was used for determining the percentage of S-phase cells

Manufacturer protocol Publication protocol 1 Relevant paper

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