Cell cycle assay human - SW480

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

FxCycle™ PI/RNase Staining Solution

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells were pretreated with incubated with dimethyl sulfoxide, sulindac (30, 60 and 120 µM), atorvastatin (0.1, 0.5, 1 µM) or Sul/Atorva. - Cells were fixed with 70% ethanol overnight.

Protocol tips
- Cells were pretreated with incubated with dimethyl sulfoxide, sulindac (30, 60 and 120 µM), atorvastatin (0.1, 0.5, 1 µM) or Sul/Atorva. - Cells were fixed with 70% ethanol overnight.

Manufacturer protocol Publication protocol 1 Relevant paper

PI/RNase Staining Buffer

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
- Cells are seeded and treated with UV-C and incubated with/without DMEM 10 μM of cisplatin for 96 h.

Upstream tips
- Cells are seeded and treated with UV-C and incubated with/without DMEM 10 μM of cisplatin for 96 h.

Manufacturer protocol Publication protocol 1 Relevant paper

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Cell fixation, washing, and cell cycle staining should be done in 12 x 75-mm polystyrene tubes

Protocol tips
- Cell fixation, washing, and cell cycle staining should be done in 12 x 75-mm polystyrene tubes

Manufacturer protocol Publication protocol 1 Relevant paper

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