Cell cycle assay human - THP-1

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

FxCycle™ PI/RNase Staining Solution

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- After 24h of cell seeding, cells were incubated with compounds 2–5 (25 μM) for different time periods in 6, 12, 24 or 96. - Cells were washed with 70% ethanol and placed for 24 h at −20 °C

Protocol tips
- After 24h of cell seeding, cells were incubated with compounds 2–5 (25 μM) for different time periods in 6, 12, 24 or 96. - Cells were washed with 70% ethanol and placed for 24 h at −20 °C

Manufacturer protocol Publication protocol 1 Relevant paper

FITC BrdU Flow Kit

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
- Cells were seeded and treated with or without recombinant human CCL2 (50 mg/ml) for 24 hours.

Upstream tips
- Cells were seeded and treated with or without recombinant human CCL2 (50 mg/ml) for 24 hours.

Manufacturer protocol Publication protocol 1 Relevant paper

Guava Cell Cycle Reagent for Flow Cytometry

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Cells were harvested, fixed and stained with Reagent on day 0, 1, 3, 5 and 7 after seeding

Protocol tips
- Cells were harvested, fixed and stained with Reagent on day 0, 1, 3, 5 and 7 after seeding

Manufacturer protocol Publication protocol 1 Relevant paper

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