Cell cycle assay human - THP-1

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

Protocol tips
- After 24h of cell seeding, cells were incubated with compounds 2–5 (25 μM) for different time periods in 6, 12, 24 or 96.

- Cells were washed with 70% ethanol and placed for 24 h at −20 °C
FITC BrdU Flow Kit

BD Biosciences

Upstream tips
- Cells were seeded and treated with or without recombinant human CCL2 (50 mg/ml) for 24 hours.
Protocol tips
- Cells were harvested, fixed and stained with Reagent on day 0, 1, 3, 5 and 7 after seeding
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms