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|- After 24h of cell seeding, cells were incubated with compounds 2–5 (25 μM) for different time periods in 6, 12, 24 or 96.
- Cells were washed with 70% ethanol and placed for 24 h at −20 °C
|- Cells were seeded and treated with or without recombinant human CCL2 (50 mg/ml) for 24 hours.
|- Cells were harvested, fixed and stained with Reagent on day 0, 1, 3, 5 and 7 after seeding
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