Cell cycle assay human - U87

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Protocol tips
- Cells were fixed for at least 2 h, and stored at −20 °C until analysis
Downstream tips
- Store tubes at 4°C protected from light prior to analyzing.

- Analyze on the flow cytometer within 1hour
Upstream tips
- Cells were seeded in serum-free condition for 24 hand made confluent with 50%.

- After 24h cells werein grown in a growth medium with or without DHMEQ
Protocol tips
- Cells are fixed for 24h at 4°C
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