Cell cycle assay mouse - 3T3-L1

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

APC BrdU Flow Kit

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
	- Cells are treated with 10 μm bromodeoxyuridine (BrdU) for 1 h. - Cells are fixed, and permeabilized with BD Cytofix/ Cytoperm Buffer containing 3–5% formaldehyde.

Protocol tips
- Cells are treated with 10 μm bromodeoxyuridine (BrdU) for 1 h. - Cells are fixed, and permeabilized with BD Cytofix/ Cytoperm Buffer containing 3–5% formaldehyde.

Manufacturer protocol Publication protocol 1 Relevant paper

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Cells are incubated with various concentrations of ARC for 24 h. - 70% ice cold ethanol is used as a fixative at −20 °C for at least 3 h.

Protocol tips
- Cells are incubated with various concentrations of ARC for 24 h. - 70% ice cold ethanol is used as a fixative at −20 °C for at least 3 h.

Manufacturer protocol Publication protocol 1 Relevant paper

FxCycle™ PI/RNase Staining Solution

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	- Cells were fixed in 75% ethanol at 4°C for 15 minutes

Protocol tips
- Cells were fixed in 75% ethanol at 4°C for 15 minutes

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox