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Found 3 matching solutions for this experiment
|- Cells are treated with 10 μm bromodeoxyuridine (BrdU) for 1 h.
- Cells are fixed, and permeabilized with BD Cytofix/ Cytoperm Buffer containing 3–5% formaldehyde.
|- Cells are incubated with various concentrations of ARC for 24 h.
- 70% ice cold ethanol is used as a fixative at −20 °C for at least 3 h.
|- Cells were fixed in 75% ethanol at 4°C for 15 minutes
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