Cell cycle assay mouse - 3T3-L1

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

APC BrdU Flow Kit

BD Biosciences

Protocol tips
- Cells are treated with 10 μm bromodeoxyuridine (BrdU) for 1 h.

- Cells are fixed, and permeabilized with BD Cytofix/ Cytoperm Buffer containing 3–5% formaldehyde.
Protocol tips
- Cells are incubated with various concentrations of ARC for 24 h.

- 70% ice cold ethanol is used as a fixative at −20 °C for at least 3 h.
Protocol tips
- Cells were fixed in 75% ethanol at 4°C for 15 minutes
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