Cell cycle assay mouse - RAW 264.7

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
	- Cells were pretreated with 100 or 200 µg/ml of EaEAC for 2 h and followed by 1 µg/ml of LPS for 24 h. - Cells were fixed with 70% ethanol at −20°C for 3 h

Protocol tips
- Cells were pretreated with 100 or 200 µg/ml of EaEAC for 2 h and followed by 1 µg/ml of LPS for 24 h. - Cells were fixed with 70% ethanol at −20°C for 3 h

Manufacturer protocol Publication protocol 1 Relevant paper

FITC BrdU Flow Kit

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
- Cells were treated with BrdU (10 μM) for 4 hours.	- Cells were treated with APC-conjugated anti-BrdU antibody

Upstream tips
- Cells were treated with BrdU (10 μM) for 4 hours.
Protocol tips
- Cells were treated with APC-conjugated anti-BrdU antibody

Manufacturer protocol Publication protocol 1 Relevant paper

Cell Cycle Assay Cell-Clock™

Biocolor

Upstream tips	Protocol tips	Downstream tips
- cells were treated with rCAMP factor (5 mg/ml) at 37 C for 24 h.

Upstream tips
- cells were treated with rCAMP factor (5 mg/ml) at 37 C for 24 h.

Manufacturer protocol Publication protocol 1 Relevant paper

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