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Found 5 matching solutions for this experiment
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- cells were fixed with 2% paraformaldehyde for 20 min and permeabilized with 1% Triton X-100 in PBS for 5 min.
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- Cells were labelled for 16h.
- Cells were denatured with 2 N HCl for 30 min at 37°C,
- Cells were blocked for 30 min by using 3% BSA in PBS. |
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- cells were fixed with 2% paraformaldehyde for 20 min and permeabilized with 1% Triton X-100 in PBS for 5 min.
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- Cells were labelled for 16h.
- Cells were denatured with 2 N HCl for 30 min at 37°C,
- Cells were blocked for 30 min by using 3% BSA in PBS. |
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RNA from total mice lungs were isolated using Fenozol (A&A Biotechnology). The quantity of ribosomal RNA and DNA contamination was examined using electrophoresis in 1% denaturing formaldehyde gel. |
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RNA from total mice lungs were isolated using Fenozol (A&A Biotechnology). The quantity of ribosomal RNA and DNA contamination was examined using electrophoresis in 1% denaturing formaldehyde gel. |
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- 1 × 104 cells/ml cells were platted in a 96 well plate.
Cells were pretreated with different concentrations of resveratrol |
- Cells were treated with 0.5 mg/ml MTT |
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- 1 × 104 cells/ml cells were platted in a 96 well plate.
Cells were pretreated with different concentrations of resveratrol |
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- Cells were treated with 0.5 mg/ml MTT |
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- Cells were incubated for 2 h at 37 °C after addition of CCK-8 reagent |
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- Cells were incubated for 2 h at 37 °C after addition of CCK-8 reagent |
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- Cells were seeded at a density of 5000 cells/cm2. |
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- Cells were seeded at a density of 5000 cells/cm2. |
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