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|- Completely thaw the 20ml size One Solution Reagent approximately 90 minutes at room temperature, or 10 minutes in a water bath at 37°C.
|- Cells were incubated with 20 μl of MTS for 3 h
|- To measure the absorbance later, add 25µl of 10% SDS to each well to stop the reaction. Store SDS-treated plates protected from light in a humidiﬁed chamber at room temperature for up to 18 hours.
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