Cell migration / Invasion cell type - A375

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 1 matching solution for this experiment

24-Well Cell Invasion Assays, Basement Membrane

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
Store all components at 4ºC and before use allow the invasion chamber plate to warm up at room temperature for 10 minutes.	Incubate for 72 hours at 37ºC in 5% CO2 atmosphere.	Measure the OD 570nm in a plate reader

Upstream tips
Store all components at 4ºC and before use allow the invasion chamber plate to warm up at room temperature for 10 minutes.
Protocol tips
Incubate for 72 hours at 37ºC in 5% CO2 atmosphere.
Downstream tips
Measure the OD 570nm in a plate reader

Manufacturer protocol Publication protocol 1 Relevant paper

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