Cell migration / Invasion cell type - HT-29

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

ORIS™ Cell Migration Assay

Platypus Technologies

Protocol tips
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 4 to 18 hours (cell line dependent) to permit cell attachment.
Protocol tips
Starve cells for 24 hours.

Incubate for 24 hours at 37ºC in 5% CO2 atmosphere
Protocol tips
Incubate at 37°C in a humidified atmosphere of 5% CO2 for 48 h
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