Cell migration / Invasion cell type - MCF-10A

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

Oris™ Universal Cell Migration Assembly Kit, 96 wells

Platypus Technologies

Upstream tips	Protocol tips	Downstream tips
Plate 15 000 cells per well	Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 8 hours (cell line dependent) to permit cell attachment.

Upstream tips
Plate 15 000 cells per well
Protocol tips
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 8 hours (cell line dependent) to permit cell attachment.

Manufacturer protocol Publication protocol 1 Relevant paper

96-Well Cell Invasion Assay, Collagen I

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
	Cover the plate and transfer to a cell culture incubator for 24 hours.	Read the fluorescence with a fluorescence plate reader at 480 nm/520 nm.

Protocol tips
Cover the plate and transfer to a cell culture incubator for 24 hours.
Downstream tips
Read the fluorescence with a fluorescence plate reader at 480 nm/520 nm.

Manufacturer protocol Publication protocol 1 Relevant paper

8 µm Chemotaxis Assays, 96-Well Format

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
	Cover the plate and transfer to a cell culture incubator for 24 hours.	Read the fluorescence with a fluorescence plate reader at 480 nm/520 nm.

Protocol tips
Cover the plate and transfer to a cell culture incubator for 24 hours.
Downstream tips
Read the fluorescence with a fluorescence plate reader at 480 nm/520 nm.

Manufacturer protocol Publication protocol 1 Relevant paper

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