Cell migration / Invasion cell type - MCF7

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
Seed 1000 cells in 24-well filter-inserts,	Cover plate and incubate for 8 hours at 37°C in a CO2 incubator (4-6% CO2).

Upstream tips
Seed 1000 cells in 24-well filter-inserts,
Protocol tips
Cover plate and incubate for 8 hours at 37°C in a CO2 incubator (4-6% CO2).

Manufacturer protocol Publication protocol 1 Relevant paper

96-Well Cell Invasion Assay, Basement Membrane

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
	For migration assay incubate cells 2–3 h at 5% CO2 and 37 °C

Protocol tips
For migration assay incubate cells 2–3 h at 5% CO2 and 37 °C

Manufacturer protocol Publication protocol 1 Relevant paper

Culture-Insert 4 Well in µ-Dish 35 mm, high

Ibidi

Upstream tips	Protocol tips	Downstream tips
Seed 70 μl of 5 × 105 cells/ml	Incubate at 37°C and 5 % CO2 for 24 h

Manufacturer protocol Publication protocol 1 Relevant paper

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