Cell migration / Invasion cell type - MHCC-97H

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

Corning® Transwell® polycarbonate membrane cell culture inserts

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
- Seed 2 × 10^5 cells per well in 200 μL serum-free medium.	- Incubate cells for 24–48 h at 37C. - Treat cells with 0.1 % crystal violet for 30 min, followed by washing with water for 30 s to remove residual dye

Upstream tips
- Seed 2 × 10^5 cells per well in 200 μL serum-free medium.
Protocol tips
- Incubate cells for 24–48 h at 37C. - Treat cells with 0.1 % crystal violet for 30 min, followed by washing with water for 30 s to remove residual dye

Manufacturer protocol Publication protocol 1 Relevant paper

QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
Seed ~1×10^5 cells	Add 20% fetal bovine serum for lower chamber Cover plate and incubate for 12 - 24 hours at 37°C in a CO2 incubator (4-6% CO2).

Upstream tips
Seed ~1×10^5 cells
Protocol tips
Add 20% fetal bovine serum for lower chamber Cover plate and incubate for 12 - 24 hours at 37°C in a CO2 incubator (4-6% CO2).

Manufacturer protocol Publication protocol 1 Relevant paper

Cultrex® Cell Migration Assay

Trevigen

Upstream tips	Protocol tips	Downstream tips
	Incubate for 16 hours at 37ºC in 5% CO2 atmosphere

Protocol tips
Incubate for 16 hours at 37ºC in 5% CO2 atmosphere

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox