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Found 3 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
Seed 2 × 10^4 cells/ml/gel |
COMET assay included ketoprofen (KP, 10 μM) as positive control. Cells to be pretreated with KP at 37 °C in a CO2 incubator (100% relative humidity and dark conditions) for 30 minutes before the irradiation step. |
The percentage of DNA damage of each sample could be calculated with the visual scoring of at least 100 DNA Comets using the following equation: [(Nclass 0 Comets × 0) + (Nclass 1 Comets × 1) + (Nclass 2 Comets × 2) + (Nclass 3 Comets × 3) + [(Nclass 4 Comets × 4) + (Nclass 5 Comets × 5) + (NClass 6 Comets × 6)]/6, where class 0 comets indicate comets with no DNA damage and class 6 comets indicate comets with maximum DNA damage. |
Upstream tips |
Seed 2 × 10^4 cells/ml/gel |
Protocol tips |
COMET assay included ketoprofen (KP, 10 μM) as positive control. Cells to be pretreated with KP at 37 °C in a CO2 incubator (100% relative humidity and dark conditions) for 30 minutes before the irradiation step. |
Downstream tips |
The percentage of DNA damage of each sample could be calculated with the visual scoring of at least 100 DNA Comets using the following equation: [(Nclass 0 Comets × 0) + (Nclass 1 Comets × 1) + (Nclass 2 Comets × 2) + (Nclass 3 Comets × 3) + [(Nclass 4 Comets × 4) + (Nclass 5 Comets × 5) + (NClass 6 Comets × 6)]/6, where class 0 comets indicate comets with no DNA damage and class 6 comets indicate comets with maximum DNA damage. |
Upstream tips |
Protocol tips |
Downstream tips |
Seed 1×10^6 cells/ml on 145-mm dishes |
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Samples can be read on an LSR II flow cytometer and analyzed using Facsdiva software. |
Upstream tips |
Seed 1×10^6 cells/ml on 145-mm dishes |
Downstream tips |
Samples can be read on an LSR II flow cytometer and analyzed using Facsdiva software. |
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