DNA isolation / purification Bacteria - Gram negative Helicobacter pylori

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

3 Matching solutions
2 Discussions

Found 2 discussions for this experiment

Discussion

5 years ago

5 years ago by Israel Lev Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Comment View 2 comments

Discussion

5 years ago

5 years ago by Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Comment View 1 comment

Share your thoughts or question with experts in your field by adding a discussion!

Found 3 matching solutions for this experiment

QIAamp DNA Micro Kit

Qiagen

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Manufacturer protocol Publication protocol 1 Relevant paper

MagNA Pure Compact Nucleic Acid Isolation Kit I

Roche Lifesciences

Upstream tips	Protocol tips	Downstream tips
For isolation of nucleic acids from relatively large organisms such as Mycobacterium, it is important to transfer the supernatant before the cells settle to the bottom of the tube.

Upstream tips
For isolation of nucleic acids from relatively large organisms such as Mycobacterium, it is important to transfer the supernatant before the cells settle to the bottom of the tube.

Manufacturer protocol Publication protocol 1 Relevant paper

Wizard® Genomic DNA Purification Kit

Promega

Upstream tips	Protocol tips	Downstream tips
	For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

Outsource experiment