DNA isolation / purification Bacteria - Gram negative Legionella

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Start discussion

Found 2 discussions for this experiment

Discussion

1 year ago

1 year ago by Israel Lev Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

1 year ago

1 year ago by Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Share your thoughts or question with experts in your field by adding a discussion!

Found 3 matching solutions for this experiment

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA
Downstream tips
If the DNA pellet is difficult to dissolve, Rehydrate DNA by incubating 1 hour at 65°C, and then leave the sample at room temperature or 4°C overnigh
Downstream tips
Wait for 48 hrs after biocide treatment before sampling 1 L of water that will be submitted to
real-time PCR analysis.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms