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1 year ago
1 year ago by Nicolai Brandt
Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?
Found 4 matching solutions for this experiment
|All solutions stored at 15 - 30°C should be clear. If precipitate is present in any reagent, warm the solution to 37°C until
the precipitate dissolves. Do not use until all precipitate has been dissolved.
|- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA