DNA quantification Blood

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

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Found 5 matching solutions for this experiment

Quantifiler™ Duo DNA Quantification Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	Place aluminum foil over the sample to protect it from light and incubate at room temperature for 5-10 minutes. Optimize incubation time to minimize signal bleeding through to other channels.

Protocol tips
Place aluminum foil over the sample to protect it from light and incubate at room temperature for 5-10 minutes. Optimize incubation time to minimize signal bleeding through to other channels.

Manufacturer protocol

Quantifiler 1 Trio DNA Quantification Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	Place aluminum foil over the sample to protect it from light and incubate at room temperature for 5-10 minutes. Optimize incubation time to minimize signal bleeding through to other channels.

Protocol tips
Place aluminum foil over the sample to protect it from light and incubate at room temperature for 5-10 minutes. Optimize incubation time to minimize signal bleeding through to other channels.

Manufacturer protocol Publication protocol 1 Relevant paper

Quant-iT™ PicoGreen® dsDNA Assay Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	To minimize photo bleaching effects, keep the time for fluorescence measurement constant for all samples. During incubation at room temperature, protected from light.

Protocol tips
To minimize photo bleaching effects, keep the time for fluorescence measurement constant for all samples. During incubation at room temperature, protected from light.

Manufacturer protocol Publication protocol 1 Relevant paper

PicoGreen® dsDNA Quantitation Reagent and Kit

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	To minimize photo bleaching effects, keep the time for fluorescence measurement constant for all samples. During incubation at room temperature, protected from light.

Protocol tips
To minimize photo bleaching effects, keep the time for fluorescence measurement constant for all samples. During incubation at room temperature, protected from light.

Manufacturer protocol Publication protocol 1 Relevant paper

Investigator Quantiplex HYres Kit

Qiagen

Upstream tips	Protocol tips	Downstream tips
special care should be taken to avoid nuclease contamination of all reagents and consumables used to set up PCR for sensitive detection of human DNA

Upstream tips
special care should be taken to avoid nuclease contamination of all reagents and consumables used to set up PCR for sensitive detection of human DNA

Manufacturer protocol Publication protocol 1 Relevant paper

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