DNA quantification Human - BMDM

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

Start discussion

Found 1 discussion for this experiment


5 years ago

5 years ago by Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

Share your thoughts or question with experts in your field by adding a discussion!

Found 3 matching solutions for this experiment

Protocol tips
To minimize photo bleaching
effects, keep the time for fluorescence measurement constant for all samples.

During incubation at room temperature, protected from light.
Qubit dsDNA HS Assay Kit

Thermo Fisher Scientific

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.
7-AAD (7-Aminoactinomycin D)

Thermo Fisher Scientific

Protocol tips
incubate cells on ice only
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms