DNA quantification Human - Hep G2

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay .

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1 year ago

1 year ago by Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Protocol tips
To minimize photo bleaching
effects, keep the time for fluorescence measurement constant for all samples.

During incubation at room temperature, protected from light.
Upstream tips
It is not necessary to fix the cells before DNA staining since the Nuclear Green CCS1 is cell- permeable
Qubit dsDNA HS Assay Kit

Thermo Fisher Scientific

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.
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