DNA transfection Mammalian cells - Immortalized cell lines PANC-1

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

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Found 4 matching solutions for this experiment

JetPrime

Polyplus transfections

Upstream tips
jetPRIME® is stable in presence of serum and antibiotics therefore you may use serum and antibiotic
containing medium during the entire experiment.
Upstream tips
Do not aliquot X-tremeGENE HP DNA Transfection Reagent; store
in the original glass vials.
Protocol tips
Do not add antibiotics to the Transfection Medium
Protocol tips
Do not add antibiotics to the Transfection Medium
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