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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-Store kit at -20°C immediately upon receipt. Kit has a storage time of 1 year from receipt, providing components have not been reconstituted.
-Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting
components.
-Do not vortex the standard during reconstitution, as this will destabilize the protein.
-When preparing your standards, it is very critical to briefly spin down the vial first.
|
-Once your standard has been reconstituted, it should be used right away or else frozen for later use.
-Be sure to discard the working standard dilutions after use – they do not store well.
|
| Upstream tips |
-Store kit at -20°C immediately upon receipt. Kit has a storage time of 1 year from receipt, providing components have not been reconstituted.
-Equilibrate all reagents to room temperature (18-25°C) prior to use. |
| Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting
components.
-Do not vortex the standard during reconstitution, as this will destabilize the protein.
-When preparing your standards, it is very critical to briefly spin down the vial first.
|
| Downstream tips |
-Once your standard has been reconstituted, it should be used right away or else frozen for later use.
-Be sure to discard the working standard dilutions after use – they do not store well.
|
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
|
| Upstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
| Protocol tips |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
|
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
|
| Upstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
|
| Protocol tips |
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
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