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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or
dilute the Detection Antibody or Streptavidin-HRP B. |
|
| Upstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
| Protocol tips |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or
dilute the Detection Antibody or Streptavidin-HRP B. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
|
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
|
| Upstream tips |
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
|
| Protocol tips |
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Store the unopened kit at 2-8°C upon receipt and when it is not in use.
-Keep microtiter plate in a sealed bag with desiccant to minimize exposure to damp air.
-All reagents should be allowed to reach room temperature (18-25°C) before use. |
-DO NOT DILUTE THE STANDARDS.
-Rinse and flick the microtiter wells 5 times with deionized or distilled water. DO NOT USE TAP WATER.
-It is recommended that the wells be read within 15 minutes following addition of Stop Solution.
|
|
| Upstream tips |
-Store the unopened kit at 2-8°C upon receipt and when it is not in use.
-Keep microtiter plate in a sealed bag with desiccant to minimize exposure to damp air.
-All reagents should be allowed to reach room temperature (18-25°C) before use. |
| Protocol tips |
-DO NOT DILUTE THE STANDARDS.
-Rinse and flick the microtiter wells 5 times with deionized or distilled water. DO NOT USE TAP WATER.
-It is recommended that the wells be read within 15 minutes following addition of Stop Solution.
|
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