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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| -Allow the microtiter plates to equilibrate to room temperature before opening the foil bags. |
-Run a standard curve with each assay.
-Drain residual wash liquid from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
-Do not cover plate with foil. |
-Once the desired number of strips are removed, immediately reseal the bag and store the plate at 2 to 8°C to maintain plate integrity.
-Store both the concentrate and the Working Wash Buffer in the refrigerator. Use the diluted buffer within 14 days. |
| Upstream tips |
| -Allow the microtiter plates to equilibrate to room temperature before opening the foil bags. |
| Protocol tips |
-Run a standard curve with each assay.
-Drain residual wash liquid from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells.
-Do not cover plate with foil. |
| Downstream tips |
-Once the desired number of strips are removed, immediately reseal the bag and store the plate at 2 to 8°C to maintain plate integrity.
-Store both the concentrate and the Working Wash Buffer in the refrigerator. Use the diluted buffer within 14 days. |
| Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
|
| Upstream tips |
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution.
-Working dilutions should be prepared and used immediately. |
| Protocol tips |
-A standard curve should be generated for each set of samples assayed.
-It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum.
-Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B. |
| Upstream tips |
Protocol tips |
Downstream tips |
| -Allow all reagents to equilibrate to room temperature (20-25°C). |
-Allow Substrate A and B to come to room temperature (20-25°C).
-Do not allow the plate to dry out between steps.
-Do not allow the substrate or SHRP to be exposed to UV light, as this may degrade it. |
- Store at room temperature (20-25°C) after mixing. |
| Upstream tips |
| -Allow all reagents to equilibrate to room temperature (20-25°C). |
| Protocol tips |
-Allow Substrate A and B to come to room temperature (20-25°C).
-Do not allow the plate to dry out between steps.
-Do not allow the substrate or SHRP to be exposed to UV light, as this may degrade it. |
| Downstream tips |
| - Store at room temperature (20-25°C) after mixing. |
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