Live / Dead assay mammalian cells - HepaRG human hepatoma

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 1 matching solution for this experiment

Upstream tips
Prepare a mix by adding 2 µL of 1 M calcein-AM stock solution and 3 µL of 1.5 M PI solution to 1 mL of Dulbecco’s modified Eagle’s medium supplemented with 2 mM of l-glutamine, 100 U/mL of penicillin and 100 µg/mL of streptomycin.
Protocol tips
Mix 100 µl of the staining solution with 200 µl of the cell suspension, and incubate the mixture for 15 minutes at 37C
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms